Enzyme catalase coursework

This is shown in the table with all the results I have gained Fig. Hydrogen peroxide is harmful and must be removed as soon as it is produced in the cell. Do not return solution to stock bottles, because contaminants may cause decomposition and the stock bottle may explode after a time.

It was very hard to insert the small 5cm3 beaker into the conical flask, and when it came to tipping it over, some of the substrate was still trapped inside the beaker.

This investigation looks at the rate of oxygen production by the catalase in pureed potato as Enzyme catalase coursework concentration of hydrogen peroxide varies. To prevent this I had to dry out the barrel and syringe before commencing the procedure.

I tried to make this as accurate as possible by keeping my eyes level with the gas syringe. Also, the reaction is so vigorous that bubbles of mixture can carry pieces of liver into the delivery tube. However, I also believed that if I halved the concentration then the rate of reaction volume of oxygen produced would also be halved, and so the rate would be proportional to the concentration.

Capillary tubes may also be used. First of all, there was an error caused by the measuring cylinder which I used to collect the volume of oxygen. Use this information as a check on the practical set-up. The oxygen oxidizes the hydroquinones and also acts as the propellant.

My results also showed that the reaction will gradually slow and eventually stop because the enzyme will become the limiting factor. Keep in brown bottles because hydrogen peroxide degrades faster in the light. A larger surface area means there are more molecules being exposed to collisions with other molecules, with sufficient energy to cause a reaction.

Hydrogen peroxide is used as a potent antimicrobial agent when cells are infected with a pathogen.

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

The catalase test is done by placing a drop of hydrogen peroxide on a microscope slide. The hand holding the tube is then tapped on the bench, moving the hydrogen peroxide down until it touches the bacteria. This is because temperature increases the rate of reaction increase due to the kinetic energy gained by the hydrogen peroxide molecules and catalase enzyme.

This means that having the same surface area of yeast in each reaction is very important in ensuring a fair test because the number of molecules exposed to collisions must be the same.

Action Of Enzyme Catalase On Hydrogen Peroxide - Lab Report Example

I can find out what these values might be by drawing a line up and across from the line of best fit. Wear eye protection and protect clothing from hydrogen peroxide.This is because as enzyme concentration increases then there is a greater probability of collision between catalase and hydrogen peroxide.

Hence more products produced in a given time.

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However the rate of reaction is constant when all substrate, hydrogen peroxide molecules have occupied the enzyme, catalase active site. Enzymes require an optimal range of temperature and pH in order to have an effective enzyme-catalyzed reaction. The enzyme used in this experiment was catalase, whose function is to prevent the accumulation of toxic levels of hydrogen peroxide formed as a by-product of cellular processes.

The enzyme catalase is responsible for the decomposition of Hydrogen Peroxide into Oxygen and Water (Palmer, Bonner, ). Previous researchers have noticed that enzymes, like catalase, perform best at their optimal temperature. Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration Class practical or demonstration Hydrogen peroxide (H 2 O 2) is a by-product of respiration and is made in all living cells.

An enzyme that is also naturally produced by the body is catalase, and this is what the liver uses to break down hydrogen peroxide. When certain factors come into play, temperature being one, catalase may not work as well. Likewise, catalase has one of the highest turnover numbers of all enzymes; one catalase molecule can convert millions of hydrogen peroxide molecules to water and oxygen each second.

Catalase is a tetramer of four polypeptide chains, each over .

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Enzyme catalase coursework
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